Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc Tris-Acetate-EDTA Buffer, TAE (50X) - 4L
The Tris-Acetate-EDTA (TAE) Buffer is a pH regulator and chelator often used in molecular biology techniques such as agarose gel electrophoresis. Supporting a pH range of 8.1-8.4, the TAE Buffer combines tris base, acetic acid, and EDTA (Ethylenediaminetetraacetic Acid) to form a solution efficient in separating nucleic acids as a running buffer, or as a component of agarose gels. This Buffer is prepared in a 50X stock solution, and can be diluted to ideal concentrations.
Sigma Aldrich Fine Chemicals Biosciences PIPES BioPerformance Certi500G
PIPES BioPerformance Certi500G
Sigma Aldrich Fine Chemicals Biosciences BICINE BioXtra 99 titratio250G
BICINE BioXtra 99 titratio250G
Teknova 1X PBS-MK with Sodium Chloride, 1000mL, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Hoefer, Inc MOPS-SDS RUNNING BUFFER 1LX10
Mighty Small precast gels Hoefer Mighty Small precast gels are high-performance mini polyacrylamide gels commonly used for protein separation.Utilizing the Bis-Tris buffer system, they effectively minimize protein modifications during electrophoresis, resulting in sharper and clearer protein bands with higher resolution. High Loading Capacity Maximum sample volume is 50 μl Good Repeatability Fully automated gel casting process, minimal batch-to-batch variation, high stability, and good repeatability High Resolution Upgraded Bis-Trissystem for clearer, sharper separation bands and higher resolution Highly Effective Maximum 40 min for electrophoresis Ready to use and easy to store No solution preparation or gel casting is required, saving time. Store at 2-8°C Safe and non-toxic No hazardous reagents need to be handled Specifications Plate Size: 10×8 cmWells: 12-well, 15-well Concentration:• Gradient gels: 4-12ĉĉĉĉĉĉĉĉĉĉMOPS-SDS Running Buffer, 1L×10ĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉ27-Mar-25 07:45:08:000 PMĉ
Cliawaived Inc Transport Tube, 3mL Phosphate Based Saline PBS (400 swabs and PBS Tubes per box)
Transport Tube, 3mL Phosphate Based Saline PBS. 102mm length vial contains 3ml of PBS solution. Includes mini-flocked swab with synthetic fibers and plastic shaft and break point at 80mm. INTENDED USEThe KaiBiLiTM Extended ViralTrans PBS is phosphate buffer saline which can maintain pH and osmotic balance, as well as provide cells with water and essential inorganic ions.SUMMARYAppropriate specimen collection and transport is important to accurate laboratory diagnosis of infectious disease. Not only personnel operating skills, but also a proper specimen collection and transport system are essential attributes for reliable diagnosis result. The KaiBiLiTM Extended ViralTrans PBS is suitable for collection, transport of clinical specimens.
FUJIFILM BIOSCIENCES INC PBS 1X-Dulbecco's Phosphate Buffered Saline Solution - Liquid
PBS 1X Dulbecco's Phosphate Buffered Saline Solution can be used to provide a buffer system to maintain cell culture media in the physiological pH range, or to irrigate, transport, or dilute fluid while maintaining cell tonicity and viability.
Quality Biological Inc DPBS without Calcium and Magnesium, 500 mL
DPBS is a balanced salt solution (BSS) ¿ Used for the handling and culturing of mammalian cells. ¿ Calcium and magnesium facilitate cell binding and clumping. ¿ DPBS without these ions can be used to wash and rinse suspended cells. ¿ Applications: DPBS serves as an irrigating, transporting or diluting fluid for mammalian cells, while maintaining cell tonicity and viability for a limited period of time. ¿ Used as a diluent of fresh red blood cells to study the trapping of red blood cell aggregate Liters ¿ Wash the wells of microtiter plates ¿ Wash cells during preparation and serial transfer. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ ¿ Max Shelf Life: 24 months
Quality Biological Inc Tris (1M), pH 9.8 1000 mL
Suitable for molecular biology protein chemistry and biochemical applications.
DNase RNase and Protease tested.
Filtered through a 0.22 µm membrane
Applications: Tris Buffer (1M), pH 9.8 is commonly used in SDS-PAGE and Electrophoresis applications.
Storage Conditions: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
DNase RNase and Protease Tested
Max Shelf Life: 36 months from date of manufacture
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
Quality Biological Inc Tris-HCl (2M), pH 6.8 500 mL
Tris-HCl Buffer is compatible with many enzymes in molecular biology (e.g. DNA modifying enzymes). Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959).
Use the Tris-HCl 0.5 M (pH 6.8) buffer for:
— stacking gels
— resolving gels
— DEAE Dextran-mediated transfection of non-adherent and adherent cells
Applications: Tris-HCl (2M), pH 6.8 is useful with SDS-PAGE and Electrophoresis.
Storage Conditions: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
DNase RNase and Protease Tested
Max Shelf Life: 36 months from date of manufacture
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
Quality Biological Inc PBS (10X), pH 7.4, Case 10 X 1000 mL
PBS is a balanced salt solution used for washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Useful in immunological applications, such as Western Blotting.
Without Calcium and Magnesium
Endotoxin and Mycoplasma tested
Storage Condition: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
Endotoxin and Mycoplasma Tested
Osmolality: 300 ± 55 mOsm/Kg H20
Max Shelf Life: 24 months from date of manufacture
Manufactured under an ISO 9001:2015 certified quality management system.
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.